I\'ve done these probelms incorrectly. Please show me how to solve them correctl
ID: 808850 • Letter: I
Question
I've done these probelms incorrectly. Please show me how to solve them correctly.
1.The molecular weight of anhydrous calcium chloride is 110.99 grams/mol. How many grams do you need for 750 ml of 250uM calcium chloride?
250 E-6 = x/110.99(1000/750)
250 E-6 = 4x/110(3)
= 0.0189g/ml
2. You have 12 mL of cells you need to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 50 uM. How much of a 30 mM stock solution of H2O2 would you add to your cells?
N1V1=N2V2
50uM(12mL)=30mM(1000)
600= 30,000N2
N2 =0.02mM
She cricled mM on this one, so I
Explanation / Answer
Hi,
A) Since, The molecular weight of anhydrous calcium chloride is 110.99 grams/mol
110.99 g/mol of anhydrous calcium chloride in 1000mL water gives you 1M solution
which means, 83.2425 g/mol (=110.99*750/1000) of anhydrous calcium chloride in 750mL water would give you 1M solution
Now, 20.811 g/mol (=83.2425*0.25/1) of anhydrous calcium chloride in 750mL water would give you 0.25 M solution
Therefore, 0.0208 g (20.811 X 10-3 g) of of anhydrous calcium chloride in 750mL water would give you 0.25mM ( 250uM) solution
B)
Given : N1 = 30 mM
V1=?
N2= 50
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