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I\'ve done these probelms incorrectly. Please show me how to solve them correctl

ID: 808850 • Letter: I

Question

I've done these probelms incorrectly. Please show me how to solve them correctly.

1.The molecular weight of anhydrous calcium chloride is 110.99 grams/mol. How many grams do you need for 750 ml of 250uM calcium chloride?

250 E-6 = x/110.99(1000/750)

250 E-6 = 4x/110(3)

= 0.0189g/ml

2. You have 12 mL of cells you need to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 50 uM. How much of a 30 mM stock solution of H2O2 would you add to your cells?

N1V1=N2V2

50uM(12mL)=30mM(1000)

600= 30,000N2

N2 =0.02mM

She cricled mM on this one, so I

Explanation / Answer

Hi,

A) Since, The molecular weight of anhydrous calcium chloride is 110.99 grams/mol

110.99 g/mol of anhydrous calcium chloride in 1000mL water gives you 1M solution

which means, 83.2425 g/mol (=110.99*750/1000) of anhydrous calcium chloride in 750mL water would give you 1M solution

Now, 20.811 g/mol (=83.2425*0.25/1) of anhydrous calcium chloride in 750mL water would give you 0.25 M solution

Therefore, 0.0208 g (20.811 X 10-3 g) of of anhydrous calcium chloride in 750mL water would give you 0.25mM ( 250uM) solution

B)

Given : N1 = 30 mM

V1=?

N2= 50

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