A phosphatase (pptase) inhibitor has an IC 50 of 1 pM for pptase1 and 1 nM for p

Question

A phosphatase (pptase) inhibitor has an IC 50 of 1 pM for pptase1 and 1 nM for pptase2 in vitro, which makes this inhibitor a potentially useful tool to specifically inhibit pptase1 in cells in order to study signaling. However, in reality, this inhibitor does not distinguish between those two phosphatases in cells: at the minimum inhibitor concentration that inhibits cellular pptase1, pptase2 is also inhibited. What does that tell you about the concentrations of pptase1 and pptase2 in cells (relative to the IC 50 ’s)?

Explanation / Answer

Answer

A phosphatase inhibitor has IC 50 1pM for pptase 1 and 1nM for pptase 2 in vitro but it doesn't inhibit cellular pptase at this concentration. But at the minimum concentration in the cells inhibits both the phosphates.

So it means inhibitor inhibits both the pptase inside the cells at a concentration higher than the IC50 values of pptases So this pattern suggests that pptase 1 concentration is more than pptase 2 because greater concentration pptase 1 need higher amount of inhibitor So this higher concentration of inhibitor may be more than the IC50 value of pptase 2.

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