A phosphatase (pptase) inhibitor has an IC50 of 1 pM for pptase1 and 1 nM for pp

Question

A phosphatase (pptase) inhibitor has an IC50 of 1 pM for pptase1 and 1 nM for pptase2 in

vitro, which makes this inhibitor a potentially useful tool to specifically inhibit pptase1 in cells in

order to study signaling. However, in reality, this inhibitor does not distinguish between those

two phosphatases in cells: at the minimum inhibitor concentration that inhibits cellular pptase1,

pptase2 is also inhibited. What does that tell you about the concentrations of pptase1 and

pptase2 in cells (relative to the IC50’s)?

Explanation / Answer

From the given information we can conclude that the concentration of pptase 2 in the cell is around 1000 times higher than the pptase 1 which make the phosphatase inhibitor to inhibit the activity of pptase 2 even at the 1 pM IC50

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