Gene expression is often assayed by measuring the level of mRNA produced from a

Question

Gene expression is often assayed by measuring the level of mRNA produced from a gene. If one is interested in knowing the amount of a final active gene product, a potential problem of this method is that it ignores the possibility of _____ a. Chromatin remodeling b. Alternative splicing c. Translational control d. Transcriptional control For the following statements Mark A for True and B for False in respect to gene expression in the lac peron. Ignore basal levels of expression. Consider the following haplotype for the bacterial la operon: I-O+Z+ There is also a plasmid with the following haplotype: I+O Z If there is no inducer present then function B-gal will be produce(T/F) Consider the following haplotype for the bacterial lac operon: I-O+Z+ There is also a plasmid with the following haplotype: I+O+z- lf glucose is present, there is no inducer, then functional B-gal will be produced(T/F) The gels shown below could explain the results on an RNA and Protein gel if there was a mutation in SRP such that it would not bind the signal peptide on a nascent polypeptide. Mark A for True and B for False (T/F)

Explanation / Answer

Ans 40. The method used to assay gene expression by measuring the levels of mRNA produced from a gene is known by the name Northern Blotting.

This is a modified version of Southern Blotting technique, developed by Dr. Edwin Southern. Northern Blot technique is also callled RNA Blot as it is used to detect specific RNA sequence in a sample.

This method has a limitation of measuring only the steady state mRNA accumulation and not RNA stability or transcriptional rates.

So, the potential problem of this method is that it ignores the possibility of (d) TRANSCRIPTIONAL CONTROL

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