Any one have experience with Confocal Microscopy/Spinning Disk Confocal Microsco

Question

Any one have experience with Confocal Microscopy/Spinning Disk Confocal Microscopy and image analysis and willing/able to answer the following questions? Thanks i advance.

4A. Once an image is digitized, it is possible to operate on each pixel of the image individually, independently of its neighbors. What is this procedure called?_________________

4B. What are digital imaging procedures that process pixels according to the value of their neighbors called?______________________________

4C. A "Neighborhood Operation" (i.e., electronic filter) that can be used to eliminate random noise and    smooth sharp boundaries and in which the digital value of the center pixel is replaced with an average of itself and its surrounding eight neighbors is called _________________________________.

4D. A "Binary" image is one in which each individual pixel is either "off" or "on", thereby creating a image which is black and white with no shades of gray.

a. True

b. False    

4E. With reference to the computer image software demonstrated in the confocal lab, it was stated that we often use a gray scale ranging from 0 to 256. In this system a value of one "1" would be very

a. white

b. dark?

c. It depends on which version of software you are using.

4F. What is the process by which an image is converted from an analog state to a digital state called? Digital to Analog conversion?

Explanation / Answer

Answer:

4A. Once an image is digitized, it is possible to operate on each pixel of the image individually, independently of its neighbors. What is this procedure called? Single-image pixel point operations

4B. What are digital imaging procedures that process pixels according to the value of their neighbors called? Local Operations

4C. A "Neighborhood Operation" (i.e., electronic filter) that can be used to eliminate random noise and    smooth sharp boundaries and in which the digital value of the center pixel is replaced with an average of itself and its surrounding eight neighbors is called Smoothing convolution filter (Spatial Averaging)

4D. A "Binary" image is one in which each individual pixel is either "off" or "on", thereby creating a image which is black and white with no shades of gray.

a. True - Correct

b. False    

4E. With reference to the computer image software demonstrated in the confocal lab, it was stated that we often use a gray scale ranging from 0 to 256. In this system a value of one "1" would be very

a. white

b. dark?

c. It depends on which version of software you are using.

4F. What is the process by which an image is converted from an analog state to a digital state called? Digital to Analog conversion? (Digitization)

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