How many grams of CaCl_2 (molecular weight = 111) would he dissolved in in water

Question

How many grams of CaCl_2 (molecular weight = 111) would he dissolved in in water to prepare 0.111M CaCla_2 solution in 2L final volume? Starting with a 20x stock solution of BSA, how many pi would be needed to prepare a: lx solution of BSA in a total of 10 mul? lx solution of BSA in a total of 20 mul? Convert The following: 3456 mm = _____m 50 g = ________ mg 45.000 mL = _______ L 15 dm =______ m 5000nm = _________ m Let's say I wanted to extract DNA from the edit of pea plants. I homogenize the pea plants using a blender, however that is not enough to break open all the cells and release its entire DNA. What additional must I take in order to break open the cell, allowing it to release its genetic information? Also what membrane must I break in order to gain access to the DNA? During our experiment "Extraction DMA from Wheat Germ Cells, " you all followed a bunch of procedures to extract the DNA. One of the procedures involved adding COLD ethanol to the wheat germ ex Why in the world did we add this COLD ethanol and what did it do the wheat germ extract? What are restriction endonucleases and for what purposes can they be used in lab? During the "Restriction Mapping of Plasmid DNA" lab What enzymes did we use? Mow many lubes did we use? What was added to each lube and WHY? Answer the following: What is agarose gel electrophoresis? What is the 3 criteria that determine the rate at which DNA moves through the agarose gel? Of these 3 criteria s, which one is negligible and why? Answer the following questions: What is TBE buffer and why is it used in electrophoresis? Why do I add loading dye to the DNA?

Explanation / Answer

Suppose the volume of 20X BSA we need to add to make 1X be A

so from the formula M1V1 = M2V2

Here M1 stock concentration

V1 volume of M1 solution to make M2 solution

M2 is the required concentration

and V2 is the final volume we require

a - 20 * A = 1 * 10

A= 10/20

A= 0.5 microlitre 20X BSA and 9.5 microlitre water

b- 20 * A = 1 * 20

A = 1 microlitre

1 microlitre 20X BSA and 19 microlitre water

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