#2: Based upon the amino acid analysis, how many peptide fragments would you exp
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Question
#2: Based upon the amino acid analysis, how many peptide fragments would you expect upon treatment of the peptide with chymotrypsin. Only give a number in the answer.
#3: Which trypsin fragment would you expect to elute last from a cation exchange chromatography column using an elution buffer with a pH = 5.5?
a) T1- CHEMFK
b) T5- LS
c) T3-NHAK
d) T2-WIMER
e) T4-CER
#4: For each fragment below, select the overall charge you would expect the peptide to have at the indicated pH:
ANSWER OPTIONS: +1, 0, -1
a) T4 at pH 7.0
b) S2 at pH 6.0
c) S4 at pH 9.0
se the following information to answer the remaining quiz questions: You are in a South American rain forest looking for naturally occurring peptides with potential as drugs. You have a mobile biochemistry lab with common reagents and enzymes, an amino-acid analyzer, gel filtration and ion-exchange chromatography, and electrophoresis. You also have an Edman Sequenator, but you have contaminated one or more of your reagents, and as a result, you cannot sequence peptides longer than about 12 residues before contaminants obscure the results. While screening extracts from the ovaries of a tropical flower, you find a peptide with potential as an antiviral agent. Your analysis is below: Amino acid analysis gives the following results: AC2E3FH2l K2LM2NR2SW. Cleavage by trypsin followed by gel-filtration chromatography gives the expected 5 products, which you sequence (shown in order of emergence from column) T1 CHEMFK T2 WIMER T3 NHAK. T4 CER T5 LS Cleavage by Staph. protease followed by gel-filtration chromatography gives the expected 4 products, which you sequence (again, shown in order of emergence from column) S1 NHAKWIME S2 MFKLS S3 RCHE S4 RCEExplanation / Answer
1) peptide sequence will be-
2) 3 peptide sequences.
3) T3- NHAK WILL ELUTE LAST.
4) T4 at pH 7.0 = zero.
S2 at pH 6.0 = +1
S4 at pH 9.0 = -1
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