When placing a DNA sample into the well of an agarose gel, its better to...circl

Question

When placing a DNA sample into the well of an agarose gel, its better to...circle your answer a) place your pipette tip as deep into the well as possible before you unload the sample. b) place your pipette tip closer to the top of the well before you unload the sample. Explain your answer to the previous question. Briefly describe one reason for adding loading dye (a.k.a. loading buffer) to your DNA sample before you place the DNA sample into your gel. If you find that your gel is secured into the casting tray with tape, you should (remove or leave in place) the tape before running electrical current through the gel. The red end of a gel box represents the positively-charged end. Based on this, you want to position your gel so that the DNA samples will travel towards the (red or black) end. You should pour the running (directly on top of qt just to the side) of the gel. Explain your answer to the previous question. Its better to leave the box of pipette tips (dosed open) throughout the entire lab period. Describe two things you can do to stabilize yourself (and the micropipette) as youre loading your sample into your gel.

Explanation / Answer

Ans 1: While loading DNA sample into the well the tip should be placed close to the top of the well before unloading the sample because holding it too deep may break the well.

So option b is correct

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