Regarding Western blot 1. You ran an SDS-page with all the correct reagents and

Question

Regarding Western blot 1. You ran an SDS-page with all the correct reagents and proper proteins with proper technique. What are TWO reasons you might not have gotten a band on a western blot? 2. Briefly explain the purpose of having 6.8 and 8.8 ph in a discontinuous gel. Regarding Western blot 1. You ran an SDS-page with all the correct reagents and proper proteins with proper technique. What are TWO reasons you might not have gotten a band on a western blot? 2. Briefly explain the purpose of having 6.8 and 8.8 ph in a discontinuous gel. 1. You ran an SDS-page with all the correct reagents and proper proteins with proper technique. What are TWO reasons you might not have gotten a band on a western blot? 2. Briefly explain the purpose of having 6.8 and 8.8 ph in a discontinuous gel.

Explanation / Answer

ANSWERS

1. TWO REASONS THAT MIGHT CAUSE NO BAND ON WESTERN BLOT.

a) IMPROPER ANTIBODY, EITHER PRIMARY AND SECONDARY.

b) LOWEST CONCENTRATION OR ABSENCE OF ANTIGEN

2. WHEN PROTEINS OF DIFFERENT MOLECULAR WEIGHT ARE TO BE ACQUIRED THEY ARE RUN AT SAME TIME BUT DIFFERNT PH

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