Need HELP!! A research team needs an antibody that binds to a protein of interes

Question

Need HELP!! A research team needs an antibody that binds to a protein of interest so that protein expression can be monitored. They use epitope tagging, adding an epitope (a sequence of amino acids) to the protein through DNA recombination. In this way, they do not need to develop an antibody to the protein, but can use a known, commercially available epitope-specific antibody. However, after following procedure, they are unable to localize the protein. What is a possible reason?

Print Calculator-Periodic Table Question 20 of 22 ncorrect sapling leaming A research team needs an antibody that binds to a protein of interest so that protein expression can be monitored. They use epitope tagging, adding an epitope (a sequence of amino acids) to the protein through DNA recombination. In this way, they do not need to develop an antibody to the protein, but can use a known, commercially available epitope-specific antibody. However, after following procedure, they are unable to localize the protein. What is a possible reason'? O O O The antigen-binding site does not recognize the antibody. The antibody is removed during RNA processing. The commercial antibodies the team used were developed from rat tissues. Only the proenzyme contains the added epitope, which is disrupted during activation Incorrect. An epitope is the specific structure within an antigen that is recognized by an antibody. The antigen-binding site on the antibody binds to the epitope of the antigen. Previous Give Up & View Solution Try Again Next about uscareerspartners priv

Explanation / Answer

Commercial antibodies are always developed specific to a particular antigen. Also during RNA processing the antibody is not removed. Hence the most possible reason could be only the proenzyme contains the added epitope, which is disrupted during activation.

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