Mandy reports that the infectious agent is a Gram-positive bacterium so that the

Question

Mandy reports that the infectious agent is a Gram-positive bacterium so that the physician can decide on a course of treatment. She then inoculates an agar plate to grow the bacteria for the coagulase test and any other tests that may be needed, a tube of Fluid Thioglycollate Medium (FTM) to determine oxygen use, and lastly, a third plate with antibiotic disks for the antibiotic sensitivity test. She then goes home for the day. The next day, Mandy is back in the lab. She sets up a control slide with two drops of saline and a test slide with two drops of saline and a loopful of the bacteria from the agar plate. She mixes rabbit plasma into each slide and rocks them gently for 10 seconds. She examines the FTM and coagulase test results. The test results show that the organism is _______. coagulase-negative and a facultative anaerobe coagulase-negative and an obligate aerobe coagulase-positive and an obligate aerobe coagulase-positive and a facultative anaerobe

Explanation / Answer

From the FTM test, we can conclude that the bacteria is an obligate aerobe, as it shows clear liquid in the anoxic zone, which is indicative of obligate aerobes.

From the coagulase test, it can be observed that some coagulation occurs on the plate, so the bacteria is coagulase-positive.

Thus, the organism is coagulase-positive and an obligate aerobe

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