In an experiment involving chromatography and spot tests: Why is it desirable to

Question

In an experiment involving chromatography and spot tests:
Why is it desirable to resolve (separate) the components of a mixture before testing for their presence? In an experiment involving chromatography and spot tests:
Why is it desirable to resolve (separate) the components of a mixture before testing for their presence? In an experiment involving chromatography and spot tests:
Why is it desirable to resolve (separate) the components of a mixture before testing for their presence? In an experiment involving chromatography and spot tests:
Why is it desirable to resolve (separate) the components of a mixture before testing for their presence?

Explanation / Answer

Separation of components is essential prior to testing for their presence to ensure that there will be no interferences in your observations. Presence of a contaminant can cause either false positive or false negative result as it can obstruct your observations. The contaminant can either mask the presence of your analyte (false negative) or alternatively, it can react similarly as your analyte (false positive). Hence, complete resolution of components is essential prior to testing.

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