Eukaryotic mRNA processing (capping, splicing, and poly A tailing) takes place i

Question

Eukaryotic mRNA processing (capping, splicing, and poly A tailing) takes place in the: A) ribosome. B) mitochondria C) endoplasmic reticulum. D) nucleus. E) nucleus and endoplasmic reticulum. Tetracycline inhibits protein synthesis by A) blocks binding of aminoacyl-tRNA to A-site of ribosome. B) blocks initiation of RNA chains. C) blocks translocation. D) prevents peptidyl transferase reaction on ribosome. E) prevents binding of mRNA to ribosome. A particular eukaryotic protein is 600 amino acids long. Which of the following could be the maximum number of nucleotides in the DNA that codes for the amino acids in this protein? A) 3 B) 100 C) 300 D) 900 E) 1, 800 Actuation of a tRNA means: A) initiation of tRNA processing. B) adding cap at the 5' end. C) transport of tRNA from the nucleus to the cytoplasm. D) interaction of tRNA with mRNA. E) insertion of amino acid into tRNA. The following statement(s) is/are true about tRNA synthetase EXCEPT: A) their number is more than 2 in a cell B) each tRNA synthetase attaches to a specific amino acid. C) belong to protein class. D) synthesis is catalyzed by RNA polymerase E) present both prokaryotic and eukaryotic cells.

Explanation / Answer

1.Eukaryotic mRNA processing takes place in the nucleus. Eukaryotes contains large no of introns or non coding sequences. The 3 main steps of mRNA processing involves-

A. 7 methyl guanosine cap are added to the 5' end of the primary mRNA transcript.

B.poly (A) tail are added to 3' ends of the transcripts, which are generated by cleavage by enzyme poly A polymerase.

C.splicing is the mechanism of removal of introns and then joining back the gaps between the exon.

The intron axon junction contains the specific sequence- exon GU-AG intron.

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