Can someone help me with this problem? I\'m not sure if its right. In the protoc

Question

Can someone help me with this problem? I'm not sure if its right.

In the protocol for this week's experiment, the need to prevent air from entering the gel bed is stressed. This is because tiny, microscopic air pockets within the gel bed can seriously disrupt buffer flow. Likewise, it is stressed that the gel material will need to be deaerated before packing the column if it has been stored in a refrigerator. Why? (will answer in lecture) Since gases are more soluble at lower temperatures, they will dissolve in the buffer during storage. When the temperature is increased to room temperature, some of the gas will come out of solution forming microbubbles in the gel material. Since gases that comprise air are more soluble at higher temperatures, taking the material out of the refrigerator will allow more gas to dissolve. To remove this gas, it is necessary to deaerate the material. After long storage, bacteria and fungi that might grow slowly in the column material might produce small amounts of gas and cause flow problems unless it is removed. The deaeration process will accelerate the process of temperature equilibration, making it possible to pour the column relatively soon after removing the material from the refrigerator.

Explanation / Answer

a. Since gas molecules are more soluble at low temperatures, they will dissolve in the in the buffer during storage. When the temperature is increased to room temperature, some of the gas will come out of solution forming microbubbles in the gel material that will affect the effeciency of the column.

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