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What would happen in the treadmilling experiment if a non-hydrolyzable analogue

ID: 94468 • Letter: W

Question

What would happen in the treadmilling experiment if a non-hydrolyzable analogue of GTP were used? The non-hydrolyzable GTP would stabilize both ends, causing treadmilling to stop. The microtubule would add monomers at both the plus and minus ends, growing in both directions. The monomers would be unable to add to the plus end, and the microtubules would shrink until they disappeared. The microtubule would treadmil until the new tubulin, with non-hydrolyzable GTP, reached the minus end, and then it would only extend at the plus end.

Explanation / Answer

The microtubule would treadmill until the new tubulin, with non-hydrolyzable GTP, reached the minus end, and then it would only extend at the plus end.

Explanation

Since, GTP is known to stabilize the microtubule filament.

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