QUESTION 6 A reversed-phase HPLC column was used to separate a mixture of compou

Question

QUESTION 6 A reversed-phase HPLC column was used to separate a mixture of compounds X and Y. The retention times for X and Y were 15.3 and 10.0 min, respectively; and the peak widths for X and Y were 0.35 and 0.78 mi, respectively. It is given that it takes 1.5 min for unretained species to move through the column. Please determine the capacity factor of the column for compound X. 15 points Save Answer 13.8 O 9.2 9.4 13.9 QUESTION 7 For the same separation as described in the last question, what's the resolution for separating X and Y? O 9.2 O 9.4 0 4.6 0 4.7 15 points Save Answer

Explanation / Answer

For question 6, use the following expression:

K = tr/to - 1

K = 15.3/1.5 - 1

K = 9.2

for question 7, use the following expression:

R = tr2 - tr1 / (w2+w1/2)

R = 15.3 - 10 / (0.35+0.78/2)

R = 9.38 or 9.4

Hope this helps

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