30% methanol-UV.pdf-Adobe Reader 2.s 50% methanol-UV.pdf-Adobe Reader *File Edit
ID: 956415 • Letter: 3
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30% methanol-UV.pdf-Adobe Reader 2.s 50% methanol-UV.pdf-Adobe Reader *File Edit View Window Help File Edit ViewWindow Help de , 2 1 / 1 7% L ; Tools Sign Comment at KB M 1 / 1 7% ; Tools Sign Comrment | | / 1 | | 66.796 | 1 | / | | | 66.7% | 04 March 2016 12:31:35 FZ1029 FZ1029 Analysis : 50% methanol Analysis : 30% methanol 7 50% methanol-UV 30% methanol 7-UV 500000 750000 400000 5000001 E 30000 200000 250000 100000 12 Minutes 6 18 2 2 4 6 8 Minutes Integration results Integration results # Peak name Rt. 1 C+T+T 2 Toluene % Area 5.39 41970751.56 90.38 4.45 3 Ethylbenzene 21.35 2399776.12 5.17 4643781241 100.00 Area Peak name Rt. Area %Area 1 Theobromine 3.26 8093851.43 34.18 2 Theophyline 3.86 8896868.67 37.57 4.49 6691577.35 28.26 15.80 2067284.73 3 Caffeine SUM 8.26 x11.69 in 8.26 x11.69 in 14:02 23/03/2016Explanation / Answer
1) for 30/70 solvent ratio,
Resolution=R=tR2-tR1/[1/2(W1+W2)]
where tR1=retention time for peak 1=3.26 min
tR2=retention time for peak 2=3.86 min
W1=peak width for peak 1=0.59 min
W2=peak width for peak 2=0.25 min
R=3.86-3.26/1/2(0.59+0.25)=0.6/0.42=1.43
R=1.43
2) for solvent ratio 50/50
tR1=retention time for peak 1=5.39 min
tR2=retention time for peak 2=15.80 min
W1=peak width for peak 1=2.05 min
W2=peak width for peak 2=1.4 min
R=15.80-5.39/[1/2(2.05+1.4)]=10.41/1.72=6.05
R=6.05
Resolution is more in case of solvent ratio 50/50 is higher ,this indicates better peak separation in case of solvent ratio 50/50 (in other words the mutual overlap of the peak is less in case of higher resolution)
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