Answering all of these questions would be much appreciated. Thank you for your h

Question

Answering all of these questions would be much appreciated.

Thank you for your help

What can you conclude if you get ALL of the following results from your PCR reactions? 1. No detectable PCR amplification of GapC DNA from your or your partners purified plant DNA template (no amplification using degenerate OR nested primers). 2. Detectable PCR amplification of GapC from the Arabidopsis gDNA template when using degenerate primers OR nested primers. 3. All of the PCR reactions that used pGAP as a template resulted in amplification of GapC DNA. 4. No detectable PCR amplification of GapC from the water only control. What can you conclude if you detect amplified DNA in all 10 of your PCR reactions (5 using degenerate and 5 using nested)? What size of DNA do you expect to see in PCR reactions that are successful?

Explanation / Answer

1) a) no detectable PCR amplification- possible reasons

primer is not working.

polymerase is not working.

dNTPs not working.

DNA is degraded.

b) either or both the primers will amplifying the gene but the quantity may vary.

c) that means this DNA sequence contains the gene of interest or positive control.

d) negative control.

2) gene has a consensus sequence.

3) 1.7 kB.

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