What numerical value of resolution is sufficient for infinitesimal overlap of tw

Question

What numerical value of resolution is sufficient for infinitesimal overlap of two Gaussian zones of equal width and height Calculate resolution of two Gaussian zones in which the width of the second zone is 2.5 times that of the first (W_2 = 2.5 W_1), the time difference between zones is 25 seconds and the standard deviation of the first zone is 3 seconds. Given the equation for a Gaussian zone: h(t) = A/sigma root2pi exp (-(t-t_R)^2/2sigma^2) where h(t) is the Gaussian function, A = peak area, sigma = standard deviation, t = time (independent variable), and t_R = retention time. what is the maximum peak height for a zone with area of 20 square seconds given the standard deviation of 5 seconds and a retention time of 2 minutes s

Explanation / Answer

1. RS= 2 (t2-t1)/W1 + W2

2. RS= 2 (t2-t1)/W1 + W2

=2 25-3 / 3.5 W1 = 12. 57 W1

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