The absorbance at 470 nm of the Fe(II)-phenanthroline complex was measured ofr s

Question

The absorbance at 470 nm of the Fe(II)-phenanthroline complex was measured ofr several different Fe^2+ concentrations in order to create a calibration curve. The absorbance at each Fe^2+ concentration was measured five times. The readings, already corrected ofr the blank solutions, are shown below. Use the averages of the replicate absorbance measurements to determine the slope and y-intercept of the best-fit straight line. Predict the Fe^2+ concentration in an unknown sample that has an absorbance of 0.178. What is the uncertainty associated with the predicted Fe^2+ concentration?

Explanation / Answer

The plot of absorbance and concentration is shown below

The slope is 0.007 /uM

at 0.178 absorbance 0.178= 0.007* concentration

Concentration =0.007/0.178= 25.42 uM

The best fi wih highest R2 value is shown below

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