An open tubular column with a diameter of 207 ?m and a stationary phase thicknes

Question

An open tubular column with a diameter of 207 ?m and a stationary phase thickness on the inner wall of 0.78 ?m passes unretained solute through in 79 s. A particular solute has a retention time of 435 s.

apling learning An open tubular column with a diameter of 207 m and a stationary phase thickness on the inner wall of 0.78 passes unretained solute through in 79 s. A particular solute has a retention time of 435 s. (b) What fraction of time does this solute spend in the stationary phase? (a) What is the partition coefficient for this solute? Number Number | total

Explanation / Answer

a) partition coefficient = It is the ratio of the amount of time a solute spends in the stationary and mobile phases

Time spent in the stationary phase = 435 - 79 = 356 s

therefore partition coefficient = 356/79 = 4.5

b) Time spent in the stationary phase = 435 - 79 = 356 s

therefore fraction of time in stationary phase = 356/ 435 = 0.82

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