Biology and Genetics

1. You have isolated a new nuclear protein, whose expression level is signficant

1. You have isolated a new nuclear protein, whose expression level is signficantly increased in cancer. Careful sequence analysis reveals the following sequence in the N-terminus …

1. You have isolated two fractions containing similar-sized vesicles by centrifu

1. You have isolated two fractions containing similar-sized vesicles by centrifugation. You suspect one fraction contains lysosomes and the other contains peroxisomes. To confirm …

1. You have just graduated from nursing school and you are considering working i

1. You have just graduated from nursing school and you are considering working in forensic nursing. a. Explain the psychiatric nurse's role in the forensic milieu. b. What are you…

1. You have performed the experiment (your practical 3, Metabolism experiment).

1. You have performed the experiment (your practical 3, Metabolism experiment). You wish to tell your friend about this experiment so they can replicate it exactly. Write a method…

1. You have prepared lipid vesicles that contain molecules of the K+ leak channe

1. You have prepared lipid vesicles that contain molecules of the K+ leak channel, all oriented so that their cytosolic surface faces the outside the vesicle. Predict how K+ ions …

1. You have purified a protein and are now trying to determine the sequence. Usi

1. You have purified a protein and are now trying to determine the sequence. Using the Edman Degradation method, you receive the results M,N,Y,I, G, R. When you digest a sample of…

1. You have purified a protein fro that causes cells in tissue culturetcteriu ti

1. You have purified a protein fro that causes cells in tissue culturetcteriu tissue culture to increase their thal D levels. You subsequently cloned a fragment of NA from bacteri…

1. You have purified a protein from a bacterium Y that causes cells in tissue cu

1. You have purified a protein from a bacterium Y that causes cells in tissue culture to increase their cAMP levles. You subsequently cloned a fragment of DNA from bacterium Y tha…

1. You have sequenced a stretch of prokaryotic DNA that is cloned into the Eco R

1. You have sequenced a stretch of prokaryotic DNA that is cloned into the Eco RI site of a plasmid. This fragment appears to be a complete transcription unit. Find the following …

1. You have the following prokaryotic DNA sequence: 5-GGACTAGACAATAGGGACCTAGAGAT

1. You have the following prokaryotic DNA sequence: 5-GGACTAGACAATAGGGACCTAGAGATTCCGAAA-3 3-CCTGATCTGTTATCCCTGGATCTCTAAGGCTTT-5 If the top strand is the template strand which sequ…

1. You hear that Fresno is threatened by future M7.1 earthquacks on Fulton Fault

1. You hear that Fresno is threatened by future M7.1 earthquacks on Fulton Fault that is concealed runs right through the center of town. This story is most a Fresno State accurat…

1. You hypothesize that a strain of plant with an unknown genotype is a heterozy

1. You hypothesize that a strain of plant with an unknown genotype is a heterozygote for the gene that controls fruit color. In this strain, the fruits are red. You cross it with …

1. You just received a dry oligonucleotide in a 2 mL tube from IDT. The oligo th

1. You just received a dry oligonucleotide in a 2 mL tube from IDT. The oligo that you ordered has the sequence TGCAGGACCAGAGAATTCGAATACA. The tube says “80.7 nmol”. How much TEz …

1. You know that PCR uses heat resistant enzymes. We used Taq polymerase, but th

1. You know that PCR uses heat resistant enzymes. We used Taq polymerase, but there are a number of heat resistant DNA polymerases that can be used. One big difference that is see…

1. You mate a normal mouse (short hair, big eyes, 5 toes, solid coat color) with

1. You mate a normal mouse (short hair, big eyes, 5 toes, solid coat color) with one that has long hair, micropthalmia (small eyes). polydactyly (extra toes), and spotted coat col…

1. You mate two haploid strains of the following genotypes: Strain 1: ADE2 ura3

1. You mate two haploid strains of the following genotypes: Strain 1: ADE2 ura3                Strain 2: ade2 URA3 Following sporulation of the diploid, select the spore phenotype…

1. You perform interrupted-mating experiments on three Hfr strains (A, B, and C)

1. You perform interrupted-mating experiments on three Hfr strains (A, B, and C). Genes are transferred (from last to first) in the following order from each strain: strain A, thi…

1. You place a plant cell in an open beaker of 0.5 M sucrose. The solution has a

1. You place a plant cell in an open beaker of 0.5 M sucrose. The solution has a s of -2.0 MPa. The plant cell has a s of -2.0 MPa and a p of +1.0. Reference: water Consider the c…

1. You plan to perform artificial DNA synthesis using oligonucleotides for const

1. You plan to perform artificial DNA synthesis using oligonucleotides for construction of a synthetic gene. The sequence of the desired gene is shown below. The synthesis reactio…

1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an

1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an aliquot of the cell suspension and counted the cells using a hemocytometer. There were 75 cells in…

1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an

1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an aliquot of the cell suspension and counted the cells using a hemocytometer. There were 75 cells in…

1. You put some potatoes in a big pot of cold water with the lid not too tightly

1. You put some potatoes in a big pot of cold water with the lid not too tightly closed. Which of the following methods to cook the potatoes would use the least amount of energy? …

1. You repeat DRT experiment with a Gram-positive rod and this is the result. Yo

1. You repeat DRT experiment with a Gram-positive rod and this is the result. Your water bath maintained a constant temperature; your dilution and plating technique is perfect. Al…

1. You should begin your speech by defining a specific problem pertaining to hea

1.     You should begin your speech by defining a specific problem pertaining to health literacy. Quantify within the definition, where possible. Consider briefly discussing the s…

1. You try to study a solution of the protein hen egg white lysozyme (HEWL), whi

1. You try to study a solution of the protein hen egg white lysozyme (HEWL), which carries a positive charge of +8e at pH 7. As a consequence, the proteins experience a significan…

1. You want to clone the gene that codes for a human enzyme you have purified, w

1. You want to clone the gene that codes for a human enzyme you have purified, which transforms a substrate X into a product Y. Which of the following approaches would be likely t…

1. You want to determine the linear order and genetic distance between three gen

1. You want to determine the linear order and genetic distance between three genes on chromosome II in Drosophila. The recessive mutant alleles are g, m, and o. You cross heterozy…

1. You want to do some studies on the structure of the Yersinia pestis (bubonic

1. You want to do some studies on the structure of the Yersinia pestis (bubonic plague) F1 proteins, which have been shown to be required for transmission of the Y. pestis bacteri…

1. You were given a sputum sample from a patient. Design an experiment on how yo

1. You were given a sputum sample from a patient. Design an experiment on how you would undergo the identification of the possible microbiological agent. You can use any previous …

1. You will be able to use turbidity as a means of determining cell growth in fu

1. You will be able to use turbidity as a means of determining cell growth in future experiments. Using the plot of absorbance versus cell count derived in this exercise, you will…

1. You will be examining the water quality of Raquette River and Woodstock Pond.

1. You will be examining the water quality of Raquette River and Woodstock Pond. In your own words, how would you define water quality? What factors would you consider to determin…

1. You will be examining the water quality of Raquette River and Woodstock Pond.

1. You will be examining the water quality of Raquette River and Woodstock Pond. In your own words, how would you define water quality? What factors would you consider to determin…

1. You will estimate the quantity of DNA present in the pUC18 DNA prep by gel ba

1. You will estimate the quantity of DNA present in the pUC18 DNA prep by gel band brightness. Compare your linear plasmid band in the HindIII digest lane to the -HindIII marker. …

1. You wish to measure the reversion rate of a gal4 mutation in haploid yeast Th

1. You wish to measure the reversion rate of a gal4 mutation in haploid yeast The gal4 mutants are unable to grow when galactose is the only carbon source so you can detect revert…

1. You wish to use dimensional analysis to estimate the event horizon\" of a bla

1. You wish to use dimensional analysis to estimate the event horizon" of a black hole, inside of which nothing can escape the gravity of the black hole (not even light). You can …

1. You work in the quality control department of a food processing plant. You ha

1. You work in the quality control department of a food processing plant. You have recently received feedback that many people have become ill and the only commonality between the…

1. You\'re studying cellular signaling through G-Protein Coupled Receptors (GPCR

1. You're studying cellular signaling through G-Protein Coupled Receptors (GPCRs). Specifically you're working on a pair of newly identified GPCRs, GPCR-X and GPCR-Y. Each binds t…

1. You\'re studying cellular signaling through G-Protein Coupled Receptors (GPCR

1. You're studying cellular signaling through G-Protein Coupled Receptors (GPCRs) Specifically you're working on a pair of newly identified GPCRs, GPCR-X and GPCR-Y. Each binds th…

1. You\'re studying cellular signaling through G-Protein Coupled Receptors (GPCR

1. You're studying cellular signaling through G-Protein Coupled Receptors (GPCRs). Specifically you're working on a pair of newly identified GPCRs, GPCR-X and GPCR-Y. Each binds t…

1. You\'ve recently discovered the sequences for two alleles for the mouse gene

1. You've recently discovered the sequences for two alleles for the mouse gene XCD, which you have creatively named XCD1 and XCD. This gene is believed to be involved in fat metab…

1. Young Woman with a Water Jug was painted by ________ . 2. Artemisia Gentilesc

1. Young Woman with a Water Jug was painted by ________ . 2. Artemisia Gentileschi painted several versions of ________ . 3. Arcadia was ________ . 4. The short-lived period known…

1. Your PhD project\'s aim is to discover a gene which can degrade \"Bisphenol A

1. Your PhD project's aim is to discover a gene which can degrade "Bisphenol A" (BA) which is a plastic related substance that is well known to be associated with a variety of dis…

1. Your center is showing signs of dehydration. He weighed 270 pounds before pra

1. Your center is showing signs of dehydration. He weighed 270 pounds before practice and now, after practice, he weighs 264. How many ounces of fluid must he drink to regain the …

1. Your consulting firm has won a remediation project at an old industrial site…

1. Your consulting firm has won a remediation project at an old industrial site…… Congratulations! Unfortunately, all the reports and records for the site were lost in a fire. Upo…

1. Your cousin complains to you that every morning when he steps outside his doo

1. Your cousin complains to you that every morning when he steps outside his door there is a smelly "deposit" left on his front steps by one his neighbor's dogs. However, he doesn…

1. Your first patient is an individual who turns out to beheterozygous for an in

1. Your first patient is an individual who turns out to beheterozygous for an inversion in chromosome . Which of thefollowing might be consequences of being heterozygous for thati…

1. Your first task will be to enumerate the number of viable cells in your stock

1. Your first task will be to enumerate the number of viable cells in your stock culture flask so you may use them for your experiment. To do so, you make a serial dilution (10 fo…

1. Your first task will be to enumerate the number of viable cells in your stock

1. Your first task will be to enumerate the number of viable cells in your stock culture flask so you may use them for your experiment. To do so, you make a serial dilution (10 fo…

1. Your friend recently traveled to Belize where he developed a severe parasitic

1. Your friend recently traveled to Belize where he developed a severe parasitic infection after being bitten by a tsetse fly. Six months later, he traveled to Bolivia and develop…

1. Your healthcare facility has asked you to explore succession planning as a po

1. Your healthcare facility has asked you to explore succession planning as a possible strategy for organizational sustainability. What will you do? To get started, research two o…