Biology and Genetics

1. You are studying the control of cAMP levels in brain slices. You have confirm

1. You are studying the control of cAMP levels in brain slices. You have confirmed that drugs such as isoproteronol that act through beta-adrenergic receptors cause a modest incre…

1. You are studying the intertidal zone of a previously unstudied island. You fi

1. You are studying the intertidal zone of a previously unstudied island. You find three species of barnacles on the rocks. Species A is most abundant in the upper intertidal zone…

1. You are studying three fruit fly mutants that affect the APS gene. All three

1. You are studying three fruit fly mutants that affect the APS gene. All three have been caused by transposon(s) jumping into genes. a) In mutant 1, the APS mRNA is not expressed…

1. You are studying two closely related species of bacteria (species A and speci

1. You are studying two closely related species of bacteria (species A and species B). When both species are grow at room temperature they form white colonies and when they're gro…

1. You are studying two types of snakes, one type has dark brown skin and the ot

1. You are studying two types of snakes, one type has dark brown skin and the other has pale tan skin. You have determined that the difference between these two snakes is due to a…

1. You are studying two types of snakes, one type has dark brown skin and the ot

1. You are studying two types of snakes, one type has dark brown skin and the other has pale tan skin. You have determined that the difference between these two snakes is due to a…

1. You are testing the rate of glucose transport into vesicles using the Na + gl

1. You are testing the rate of glucose transport into vesicles using the Na + glucose pump. A. in experiment 1, you employ liposomes that have the pump in the same orientation as …

1. You are the general counsel for a multinational biotechnology company. Your C

1.You are the general counsel for a multinational biotechnology company. Your CEO has asked for a detailed description of what the company must do to ensure that it is in complian…

1. You are the general counsel for a multinational biotechnology company. Your C

1.You are the general counsel for a multinational biotechnology company. Your CEO has asked for a detailed description of what the company must do to ensure that it is in complian…

1. You are the head of a bioanalytical laboratory in a hospital. Doctors asked y

1. You are the head of a bioanalytical laboratory in a hospital. Doctors asked you to check the presence of Hepatitis virus (the dsDNA-based virus) in new patients arriving in the…

1. You are trying to purify a protein that has a total ratio of hydrophobic to h

1. You are trying to purify a protein that has a total ratio of hydrophobic to hydrophilic amino acids of 70:30 so you decide to purify the Lysate using HIC. During the process th…

1. You are working for a company, “Your DNA” that provides individuals with thei

1. You are working for a company, “Your DNA” that provides individuals with their genome sequence that is printed on a tee shirt. You have sequenced the DNA of an individual and i…

1. You are working in a hospital and a patient was brought in with purple rash c

1. You are working in a hospital and a patient was brought in with purple rash covering their body. You need to explain to the others that this patient has a Neisseria meningitidi…

1. You are working in a lab and have been given a project which requires that yo

1. You are working in a lab and have been given a project which requires that you clone the promoter of the "gene A" into the "multiple cloning site" of Vector X. You will then cl…

1. You are working in a pharmaceutical lab and your project is to develop a drug

1. You are working in a pharmaceutical lab and your project is to develop a drug that will treat itchy red skin that occurs as an immune response to poison ivy. All of the current…

1. You are working in the intensive care unit, and your patient’s heart rate sud

1. You are working in the intensive care unit, and your patient’s heart rate suddenly decreases from 88 to 50 beats per minute. What may be some of the reasons for the decreased h…

1. You are working on a research project involving a novel transcription factor

1. You are working on a research project involving a novel transcription factor that regulates gene expression specifically in muscle cells (called MUSC1). You have already done a…

1. You are working on an experiment in yeast and wish to know which polymerase t

1. You are working on an experiment in yeast and wish to know which polymerase transcribes your favorite gene. Your species cannot be transformed- so you can’t make mutations in t…

1. You breed two rose species together one has large red flowers and has thorns,

1. You breed two rose species together one has large red flowers and has thorns, the other has small yellow flowers and is thornless. the F1s all have small red flowers and thorns…

1. You calculate a Ch-Square value of 10.02 for data with 3 degrees of freedom (

1. You calculate a Ch-Square value of 10.02 for data with 3 degrees of freedom (df). What statement(s) can you make with respect to your null hypothesis? [1 can reiect the nuti hu…

1. You conduct a point count sample on soil particles and you find the following

1. You conduct a point count sample on soil particles and you find the following: 116 Sand 122 Clay Silt Pore Space 15 a. What is the percent of porosity? b. What is the relative …

1. You create an artificial cell in a test tube. This artificial cell consists o

1. You create an artificial cell in a test tube. This artificial cell consists of a cytoplasm (i.e. the inside of the cell), a lipid bilayer, and the outside. The cytoplasm contai…

1. You decide to measure the Vm of the membrane below to verify your calculation

1. You decide to measure the Vm of the membrane below to verify your calculation. You place an axon in a dish containing a solution that matches the table above. Next you put your…

1. You discover a new antimicrobial that inhibits protein synthesis by binding t

1. You discover a new antimicrobial that inhibits protein synthesis by binding to the mRNA. What class does this new drug most likely belong in? macrolides. 2.Select all of the ac…

1. You discover a small protein containing only 10 amino acids, called interleuk

1. You discover a small protein containing only 10 amino acids, called interleukin-87 (IL-87), that is involved in modulating the immune response. You are interested in purifying …

1. You discover a small protein containing only 10 amino acids, called interleuk

1. You discover a small protein containing only 10 amino acids, called interleukin-87 (IL-87), that is involved in modulating the immune response. You are interested in purifying …

1. You discovered a new organism unknown to science. As you are dissecting it, y

1. You discovered a new organism unknown to science. As you are dissecting it, you notice that the digestive track is relatively short with very small or reduced additional chambe…

1. You fill each of three (10 ml) dialysis tubes with one of the following subst

1. You fill each of three (10 ml) dialysis tubes with one of the following substances A. 2% by weight of glucose(MW 180) in RO water B. 2% by weight bovine serum albumin (MW 66000…

1. You fill each of three (10 ml) dialysis tubes with one of the following subst

1. You fill each of three (10 ml) dialysis tubes with one of the following substances: a. 2% by weight of sucrose (MW 342.297) in RO water. b, 2% by weight bovine serum albumin (M…

1. You find a sediment that is well rounded, well sorted and the particles are s

1. You find a sediment that is well rounded, well sorted and the particles are small What is the mode of transport? (water or ice?) What is the distance from source? (near/medium/…

1. You found a new species of garden pea and have decided to repeat Mendel\'s ex

1. You found a new species of garden pea and have decided to repeat Mendel's experiments. You have obtained two true-breeding pea plants that are tall (T) with yellow pods (g), an…

1. You have a cell and you know the concentrations of some ions, but not the oth

1. You have a cell and you know the concentrations of some ions, but not the others. Na] IK-1 CI-] (A-1 Calculate the concentration of the unknown ions based on the following: Int…

1. You have a cell and you know the concentrations of some ions, but not the oth

1. You have a cell and you know the concentrations of some ions, but not the others. Intracellular Fluid] ? mM 142 mM ? mM 127 mM [Extracellular Fluidl INa'] IK'] [CI-I A-1 135 mM…

1. You have a circular piece of DNA that can be cleaved by the restriction nucle

1. You have a circular piece of DNA that can be cleaved by the restriction nucleases EcoRI, HindIlI, and Notl at the positions indicated on the "plasmid map" below. Considering th…

1. You have a mystery protein that you suspect is a regulator of actin polymeriz

1. You have a mystery protein that you suspect is a regulator of actin polymerization. investigate this, you acquired a few reagents: pyrene-labeled actin monomers at 1 M concentr…

1. You have an oil reservoir with the following properties a. Depth 8500 ft b. I

1. You have an oil reservoir with the following properties a. Depth 8500 ft b. Initial reservoir pressure 29 c. Reservoir Temperature 175 F d. Porosity 10% e. Total reservoir area…

1. You have been given a 9 residue peptide of biological interest to study. You

1. You have been given a 9 residue peptide of biological interest to study. You determine that the first thing you need to know is the sequence. Given below is the data you need t…

1. You have been provided with seven partial DNA sequences which originated from

1.       You have been provided with seven partial DNA sequences which originated from seven different organisms. Each of these sequences is from a gene that codes for a b-globin …

1. You have begun working in a lab studying drugs that inhibit metabolism. Your

1. You have begun working in a lab studying drugs that inhibit metabolism. Your lab has discovered compounds naturally occurring in foods that inhibit acyl-carnitine/carnitine tra…

1. You have cloned the gene sequence of the novel eukaryotic protein you have na

1. You have cloned the gene sequence of the novel eukaryotic protein you have named Protein Bob (2,100 bpin length). You have used PCR to amplify up the Protein Bob gene from your…

1. You have cloned the gene sequence of the novel eukaryotic protein you have na

1. You have cloned the gene sequence of the novel eukaryotic protein you have named Protein Bob (2,100 bp in length). You have used PCR to amplify up the Protein Bob gene from you…

1. You have constructed a cell strain whose actin is fused with a photo-activate

1. You have constructed a cell strain whose actin is fused with a photo-activated greern fluorescent protein, PA-GFP. When PA-GFP is first expressed, it starts as non-fluorescent …

1. You have constructed a cell strain whose actin is fused with a photo-activate

1. You have constructed a cell strain whose actin is fused with a photo-activated green fluorescent protein, PA-GFP. When PA-GFP is first expressed, it starts as non-fluorescent. …

1. You have determined there is a mutation in a strain of yeast in which a compo

1. You have determined there is a mutation in a strain of yeast in which a component of TFIID retains full binding activity with DNA at an elevated temperature but can no longer m…

1. You have discovered a novel sigma factor in coli cells called ?25 Based on da

1. You have discovered a novel sigma factor in coli cells called ?25 Based on data from a DNase foot-printing assay you have determined that ?25 binds to consensus sequences at po…

1. You have discovered a novel sigma factor in coli cells called ?25 Based on da

1. You have discovered a novel sigma factor in coli cells called ?25 Based on data from a DNase foot-printing assay you have determined that ?25 binds to consensus sequences at po…

1. You have discovered a novel sigma factor in coli cells called ?25 Based on da

1. You have discovered a novel sigma factor in coli cells called ?25 Based on data from a DNase foot-printing assay you have determined that ?25 binds to consensus sequences at po…

1. You have discovered a novel sigma factor in coli cells called ?25 Based on da

1. You have discovered a novel sigma factor in coli cells called ?25 Based on data from a DNase foot-printing assay you have determined that ?25 binds to consensus sequences at po…

1. You have flowering plants where yellow flowers (Y) are dominant to white, tal

1. You have flowering plants where yellow flowers (Y) are dominant to white, tall plants (T) are dominant over short plants, and round seeds are recessive to oblong (O). You grow …

1. You have identified a protein factor that when added to in vitro protein synt

1. You have identified a protein factor that when added to in vitro protein synthesis reaction increases the incorporation of 35S-methionine from 4 pmoles/minute to 8 pmoles/min. …