What problem will arise if the level of the solvent in the developing chamber is

Question

What problem will arise if the level of the solvent in the developing chamber is higher than the spot that initially applied to the TLC plate? Draw the chemical structure of the following compounds, and then arrange these compounds in order of creasing Rf on a TLC plate (1 = lowest Rs 4 = highest Rf). Explain why the compounds will be arranged that order. acetic acid, 2-octanone, decane, butanol t problem will arise if the TLC plate remains in the developing chamber too long, so that the solvent front surpasses the top? 5. What will be the appearance of the paper chromatogram if a solvent of very low polarity is used to elute a polar analyte? Explain. 6. Which of the following pairs of compounds would be most easily separated by thin layer chromatography: octanol and 1-octene, hexadecane and octadecane, or chlorobenzene and bromobenzene? Provide a clear explanation for your choice. 7. Briefly explain how thin layer chromatography could be used to aid in the identification of a compound:?

Explanation / Answer

Question Number 3:

Acetic acid structure (CH3-COOH)

2-Octanone (CH3-CO-CH2-CH2-CH2-CH2-CH2-CH3)

Decane (CH3-(CH2)10-CH3)

Butanol (CH3-CH2-CH2-CH2-OH)

The smallest Rf value will be given by the most polar substance, which in this case is acetic acid. The order of polarity then changes from butanol, 2-octanone and in the last Decane with all Carbon bonds making it the least polar of the lot

Highest Rf value = Decane

Lowest Rf value = Acetic Order

Hence the correct order will be

Acetic Acid < Butanol < 2-Octanone < Decane

Question Number 4:

The chemicals will move up the TLC plate along with the solvent. If the solvent reaches the top of the plate after keeping it so long, then the chemical will still continue to move up. Due to this phenomena, all the chemicals will merge at the top of the plate and it will become difficult to seperate the chemicals from each other

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