Compare the pellets obtained after salting out with different concentrations of

Question

Compare the pellets obtained after salting out with different concentrations of salt. Explain why did you observe this difference ? Prepare a protein solution by pouring 30 ml of egg albumen (un-pasteurized egg white) into a beaker and dilute to 60 ml with distilled water. Prepare (5) times 10.0 ml aliquots of the dilute egg solution. Slowly add Ammonium Sulfate to each aliquot (w/v) for final concentrations of 0, 15, 30, 45 and 55% (w/v) (use ammonium sulfate precipitation table2.3.). Mix thoroughly to dissolve the salt solution (approx. 10 minutes), but do not denature the protein (the presence of foam indicates denaturation). A hazy precipitate may form. Take 2 ml of the salt + protein mixture in to clean 2 ml centrifuge tubes. Centrifuge each salt/albumen solution @ 14000 rpm for 10 minutes. Some of the tubes will have two distinct phases: a liquid "supernatant" and a solid "pellet" on the bottom. Carefully transfer the supernatant of each tube (into a labeled

Explanation / Answer

Q1.

Note that different salts will increase the boiling point of water, therefore, higher temperature/heat is achieved

for

ammonium sulfate = (NH4)2SO4 --> 3 ions, 2NH4+ and SO4-2, then i effect = 3

note that the more concentrated, the higher the boiling point, since it also depends on concentration of salt.

Then

55% of w/v solution will have the highest boiling point

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