Calculate the number of theoretical plates, N, for each peak I am so confsued, c

Question

Calculate the number of theoretical plates, N, for each peak

I am so confsued, can someone please help me!

Consider the data below for a liquid chromatography separation of four compounds, A, B, C, and D on a 45.0 cm Column Calculate the number of theoretical plates, N, for each peak. Retention Width at the Time (min) Base (min) A 5.09 0.447 Number Number 12.3 1.04 C 14.7 1.12 D 23.1 1.93 Number Number What is the average number of theoretical plates, N for the column? Number avR What is the plate height, H, for the column? Number cm

Explanation / Answer

Number of theoritical plates= 16 X (retention time)2/ (Width at Base)2

NA= 2076 plates

NB= 2238 Plates

Nc=2756 Plates

Nd=2292 Plates

Average number of theoritical columns=2340

Hight of the column= Length of the column/Average number of plates= 45.0/2340=0.019 cm

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