33) Which of the following statements regarding the Entner-Doudorolf pathway is

Question

33) Which of the following statements regarding the Entner-Doudorolf pathway is TRUE 33) A) It involves glycolysis B) NADH is generated. C) ATP is generated. D) It involves the pentose phosphate pathway E) NADH and ATP are generated 34) An enzyme, citrate synthase, in the Krebs cycle is inhibited by ATP. This is an esample of all of the) following EXCEPT A) allosteric inhibition. C) competitive inhibition B) noncompetitive inhibition. D) feedback inhibition Figure 6.1 35) In Figure 6.1, which line best depicts a facultative anaerobe in the absence of O2? 35) A) a B)b C)C 36) If cells are grown in media containing amino acids labeled with radioactive nitrogen (15N), most of 36) radioactivity will be found in the cells A) DNA. B) proteins C) phospholipids D) DNA and proteins. E) DNA and phospholipids 37) Pathogenic bacteria isolated from the respiratory or intestinal tracts of humans are 37)_ A) facultative anaerobes that require reducing media for growth. B) capnophiles that prefer highly oxygenated growth conditions C) strict aerobes that grow best in reducing media D) capnophiles that grow best in carbon dioxide incubators. E) strict aerobes that grow best in candle jars. 385 The biosaséety level GSL) for a clinical microbiology laboratory working with potentaly arborne 3 pathogens, such as tuberculosis bacteria, is A) BSL-1. B) BSL-2 C) BSL-3 D) BSL-4

Explanation / Answer

33. (E) NADH and ATP are generated. In this pathway, for each utilized glucose molecule, 1 molecule of ATP and 1 molecule NADH and 1 molecule of NADPH is generated to finally convert glucose into pyruvate.

34. (C) Competitive inhibition. Because in competitive inhibition, binding of an inhibitor prevents binding of the target molecule of the enzyme.

35. (B) The line b depicts the growth of a facultative anaerobe in absence of O2. As in absence of O2, facultative anaerobes show an intermediate growth, not an optimal one.

36. (D) DNA & Proteins. Proteins and DNA would have Nitrogen in their structures but phospholipids do not have nitrogen. So, radioactive nitrogen can only be found in both DNA and Protein.

37. (D) Capnophiles, that grow best in carbon dioxide incubators. Campylobacter sp. is a common example of capnophile.

38. (C) BSL-3. The specifications and guidelines of different biosafety levels are well demarked by World Health Organisation.

39. (D) 54,000 cells. The third plate has got a dilution level of 10-3, therefore to get the actual number of viable cells in the sample, we have to multiply the cell count of that plate (54) by 103, hence it is 54,000.

40. (A) Hypertonic environment. Hypertonic environment does not allow microorganisms to grow, this technique is utilized in making pickles.

41. (A) Determines the number of viable cells. That is the only advantage of standard plate count among the given options. Standard plate count technique requires some hours to days for viable cells to appear on agar plates.

42. (C) C phase of the growth curve - is called the stationery phase which comes after the exponential or log phase of growth in bacteria.

43. (B) pH5. This is the most optimum pH range for fungal growth.

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