Your research group was asked to investigate an enzyme, two substrates and a coe

Question

Your research group was asked to investigate an enzyme, two substrates and a coenzyme given to you by a team conducting experiments to regulate their protein. Glutamate dehydrogenase (EC 4.2.6.3) is important not only in amino acid synthesis but also in the metabolism of alpha-ketoglutarate. The team conducted some experiments and found that the Km for substrate-1 went from 1.2x10^-2 units to 4.1x10^-3. This change was observed when guanosine triphosphate (a known effector) was added to the reaction mixture. An undergraduate student completed the experiment and sound that the Michaelis constant for substrate-2 went from 2.4x10^-2 units to 3.9x10^2 units after the effector was added. Also, the experiment showed that the maximum velocity did not change for both substrates.

Without GTP in the reaction, the enzyme-substrate complex:

Will form with substrate-1 followed by substrate-2

Will form with substrate-2 followed by substrate-1

Will form with both substrate simultaneously

Will form with the effector

Explanation / Answer

Without the Guanosine triphosphate, the enzyem substrate complex will fom with the both substrates simultaneoulsy because the vakue of the michaelis constant has no relevant relation with the formation of the enzyme-substrate complex and is independent of the number and types of the substrates for the reaction.

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