Review-DNA structure (10 pts) 1. You have a sample that includes equimolar amoun

Question

Review-DNA structure (10 pts) 1. You have a sample that includes equimolar amounts of protein, dsDNA, ssDNA and typical eukaryotic mRNA. You want to separate the 4 components of the mixture in 3-4 steps, without destroying any of the molecules of your sample. At the end of your separation you must have one and only chemical species in each tube (one tube with dsDNA, one with ssRNA, etc.) Briefly describe the sequence of steps that you would take using a diagram. To get full credit you need to: . number the steps sketch what you are doing, clearly and concisely explain what you are doing in words. . ·

Explanation / Answer

First the mixture passes through Poly-T affinity Column Chromatography. Here the mRNA separated from the rest of the mixture . Then the elites mixture subject to Gel filtration chromatography, where protein become separated and remaining eluted out. Then the rest of the mixture is subject to Agarose gel electrophoresis. Where 2 bands formed. The band near to Wells is SS DNA and far to Wells is DS DNA. From gel we can separate them

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