Can you calculate the map distance between ad and trp? Which are the PD, NPD and

Question

Can you calculate the map distance between ad and trp?

Which are the PD, NPD and T types of octads?

A Neurospora strain that required both adenine (ad) and tryptophan (trp) for growth was mated to a wild-type strain and this cross produced seven types of ordered tetrads in the following frequencies: Spore Pair 1:ad trp adad trp ad trp ad trp adadtrp Spore Pair 2:ad trp adadtrp trp+ + Spore Pair 3:trp+trp ad ad trp adad trp Spore Pair 4: trptrp ad+ Type (1) 63 (2) 3 (3) 15 (4)9 (5) 3 (6) 1 (7) 6 What is the centromere -gene distance for ad and trp?

Explanation / Answer

For ordered tetrad,

centromere-gene distance for ad and trp

= ((1/2* Asci with II division segregation pattern) / Total number of Asci ) * 100

= ((1/2* (3+15+9+3+1+6)) / 100) * 100

= 18.5 m.u.

P.D = Parental ditype. For instance: A/B ; a/b; A/B ; a/b

NPD= Non-parental ditype. For instance: A/b ; a/B; A/b ; a/B

T= Tetratype. For instance, A/B ; a/b; A/b ; a/B

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