A protein called CVF75 is synthesized as a pre-proportion in the beta cells of t

Question

A protein called CVF75 is synthesized as a pre-proportion in the beta cells of the pancreas. Its pre-peptide is cleaved off after it enters the ER lumen. To define the cellular location at which its propertied is removed, you have prepared two antibodies: one that is specific for pro-CVF75. and one that is specific for CVF75. You have tagged the anti pro-CVF75 antibody with a red and the anti CVF75 antibody with green. so that you can follow them independently in the same cell. When you incubate a pancreatic beta with a mixture of your two antibodies. you obtain the results shown m the table below. In what cellular compartment is the removed from Explain your answer based on the results presented in the table below. Explain each result observed.

Explanation / Answer

The pro-CVF75 peptide is cleaved off at the endoplasmic reticulum (where it first turns red) whereas the CVF75 is fully cleaved after it leaves the Golgi and has already been packaged into an immature secretory vesicle. The immature vesicles turn from red to yellow to green, indicating the final cleavage as maturation occurs.

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