A protein biochemist attempted to determine the amino acid sequence of a decapep

Question

A protein biochemist attempted to determine the amino acid sequence of a decapeptide. Use the results from the trypsin, chymotrypsin, and cyanogen bromide treatments to suggest the amino acid sequence of this decapeptide. Trypsin digestion gave two fragments, containing the following residues (not in order):

T1: Ala, Arg, Phe, Pro, Thr, Trp, Tyr
T2: Lys, Met, Val

Chymotrypsin digestion gave three fragments with the following residues (not in order):
CT1: Ala, Arg, Phe, Pro
CT2: Thr, Trp
CT3: Lys, Met, Tyr, Val

N-terminal analysis of the CT1 tetrapeptide indicated that the N-terminal amino acid was Ala.

The cyanogen bromide gave only one single amino acid, methionine, and a nonapeptide.

What is a possible sequence of the decapeptide? Use three-letter abbreviations in your answer, and add dashes between residues.

Explanation / Answer

Possible sequence of the decapeptide = Met-Val-Lys-Tyr-Thr-Trp-Ala-(Arg,Pro)-Phe

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