What can conclusions can be made? How does pH affect this experiment? What would

Question

What can conclusions can be made? How does pH affect this experiment? What would be the PI here? What amino acids would likely react where the problem states there is a change in the picture? Are there any purification techniques that can be done to separate the protein of interest? I'm trying to get as much information that can be related to this problem.

pH pH I perform a 2D gel electrophoresis of proteins from the eggs of two different species of birds. I then immunoprecipitate the protein marked "X" in species 1 using an antibody specific to that protein. The same antibody immunoprecipitates the protein marked "Y" in species 2. After treating the purified proteins with Glycosidase, they run identically on the 2D gel. Sequencing shows that X and Y have identical amino acid sequences. 13 13 300 ?! 10 E 10 Species 1 Species 2

Explanation / Answer

on looking at the results, there are two possibilities. One is that the content of the protein in two different species of the bird may be same as they might have shared the common ancestors. Another thing is that there are certain functional proteins that are common in all the eggs. these proteins are the basic proteins needed for the functioning of the developing baby inside the egg.

pH plays an important role in separation. The proteins in the 2D gel electrophoresis are separated on the basis of two factors. one is mass another one is an isoelectric point. The pH at which particular molecule has no weight is called its isoelectric point.

For every protein, there will be different PI. you have to identify the protein and then you will be able to know its PI easily. other directly measure the PI of your protein. The reaction of amino acids depending upon the enzyme used on the protein.

chromatography. HCPL and many other techniques can be used to separate protein of your interest.

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