These are questions from a study guide I already completed but wanted to double
ID: 210549 • Letter: T
Question
These are questions from a study guide I already completed but wanted to double check my answers for. Thank you!!
What is the role of the sigma factors in prokaryotic transcription? What is the result of changing sigma factors within a cell?
How is a consensus sequence determined? What can you say about the strength of a promoter in relation to the consensus sequence?
Diagram a prokaryotic transcript—what information is included in the transcript? In what ways would if differ from a eukaryotic primary transcript?
What is the difference between positive and negative regulation, how is each defined? What is the difference between inducible and repressible gene regulation. What types of genes are regulated by induction vs. repression?
Why are the target sequences for DNA binding proteins frequently inverted repeats?
Which RNA polymerase in eukaryotes is responsible for transcribing protein coding genes?
Explanation / Answer
1st question answered
Sigma factor is a protein. It aids specific binding of the RNA polymerase (the enzyme the performs transcription- copies DNA code into mRNA code) to the promoter sequence in the gene and hence is responsible for the initiation of the Transcription event. Change in sigma factors would cause transcriptional blockage and hence subsequently the death of the prokaryote.
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