A correctly performed sequencing run is shown to the right. A G cT Which of the

Question

A correctly performed sequencing run is shown to the right. A G cT Which of the following should be included in the reaction run in lane 1 of this gel? (check all that apply): (2 pts) dATP ddATP (dideoxy) ddGTP (dideoxy) ddCTP (dideoxy) ddTTP (dideoxy) 1. PCR and Sequencing Reactions are similar in which of the following ways? GTP dCTP dTTP (check all that apply) (2 pts) They require 2 primers They require DNA polymerase They require dideoxy NTPs (ddNTPs) They require deoxy NTPs (dNTPs) They result in exponential amplification of target DNA 2. Huntington's Disease is an autosomal dominant condition caused by expanding trinucleotide CAG repeats. (2 pts each) i.The varying number of CAG repeats that contribute to Huntington's Disease are a polymorphism belonging to which of the following groups?: A. InDel B. CNV C. SNP D. Microsatellite Repeat/SSR

Explanation / Answer

Lane 1 of the gel requires ddATP may be radioactively or flouresently labelled for the detection in automated sequencing machine.

dATP deoxynucleotide triphosphate : For the lane 1 adenine is present so dATP would be used.

1.Both the process of PCR and sequencing requires dNTPs for the extension of DNA.

Sequencing requires only one primer while PCR requires two primers.

Sequencing requires DNA polymerase while PCR requires taq DNA polymerase which can high melting temperature.

Only ddNTPs are required in sequencing which are terminating bases with flouresently tags.

2. Huntington's Disease : Microsatellite Repeat/ SSR - a Microsatellite triplet repeat in a coding region .It is a neurodegenerative disorder.

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