8. The following reagents are often used in protein work: CNBr Performic acid Ph

Question

8. The following reagents are often used in protein work: CNBr Performic acid Phenyl isothioyanate Dansyl chloride Chymotrypsin Mercaptoethanol Trypsin SDS Ethanol Urea 6N HCI Triton X-100 Which is best suited for the procedures described below: a) Determination of the amino acid sequence of a small peptide. b) Hydrolysis of peptide bonds on the carboxyl side of aromatic residues. c) Cleavage of peptide bonds of the carboxyl side of methionines. d) Reversible denaturation of a protein lacking disulfide bonds. e) Irreversible denaturation of a protein containing disulfide bonds.

Explanation / Answer

Determination of the amino acid sequence of a small peptide.
The answer is : Phenyl isothiocyanate

Hydrolysis of peptide bonds on the carboxyl side of aromatic residues.
The answer is : chymotrypsin

Hydrolysis of peptide bonds on the carboxyl side of methionines.
The answer is : CNBr

Reversible denaturation of a protein devoid of disulfide bonds.
The answer is : urea and -mercaptoethanol

irreversible danaturation of a protein containing disulfide bonds

Related Questions

Navigate

• Browse (All)
• Browse 8
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.