As a graduate student, by fusing protein-X with GFP, you show that it is located
ID: 217296 • Letter: A
Question
As a graduate student, by fusing protein-X with GFP, you show that it is located entirely in nucleus. But you wonder if protein-x isa true nuclear protein or a shuttling protein that spends most of its time in nucleus. You are unsure how to resolve this issue. You advisor suggests that you make a heterkarvon by fusing cells that are expressing GFP tagged protein-x with the cells that are not expressing this protein. A. What results would you expect if protein were a true nuclear protein? B. What results would you expect if protein-x were a shuttling protein?Explanation / Answer
A. If the Protein X is truely a nuclear protein after the generation of Heterokaryon the localization of the protein-X will be in the nuclues that express the protein-x but the nucleus that does not express protein-x will not show the presence of protein-x. Because a true nuclear protein will not transported to the cytoplasm and again to the nucleus. As the transport is restricted it will remain in the true nucleus
B. if the protein-x is a shuttling protein, after the generation of heterokaryon, the localization of the protein-x will be seen in both the nucleus. As a shuttling protein transport to and fro form nucleus to cytoplasm the protein have chance of transporting to the non-expressing nucleus through its nuclear pore.
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