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What would happen to the DNA sequence if the PCR mixture contained ddNTPs and no

ID: 252250 • Letter: W

Question

What would happen to the DNA sequence if the PCR mixture contained ddNTPs and not dNTPs? View Available Hint(s) All products would be one nucleotide longer than the primer. Products of various sizes would be synthesized. OAll products would be the same size as the template strand. All products would be four nucleotides longer than the primer Submit PartE Companies like Mumina or 23andMe say they will sequence your whole genome if you send in a sample containing your cells. What technique might be used for whole-genome sequencing? cDNA library Shotgun sequencing PCR Dideoxy sequencing Submit Request Answer

Explanation / Answer

1. All products would be one nucleotide longer than the primer.

ddNTPs inhibit the process of elongation. It does not have free 3'OH group to form phosphodiester bond.

E. Shotgun sequencing.

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