A biology 207 student sub cloned the promoterless, GFP gene from pCam R -GFP int

Question

A biology 207 student sub cloned the promoterless, GFP gene from pCamR-GFP into pUC18-207 using a double digest. They used competent, prototrophic DH5?E. coli cells in their transformation protocol and plated the transformation reaction onto minimal medium that contained additional supplements to help them screen and select for their desired transformants, which are transformed E. coli cells that express the GFP gene. All experimental procedures were followed such as incubation times etc. Note that GFP encodes for a protein that fluoresces green. All you need to answer the questions that follow are the diagrams of the plasmids below and the information throughout this problem.

followed such as incubation times etc. Note that GFP encodes for a protein that fluoresces green. All you need to answer the questions that follow are the diagrams of the plasmids below and the information throughout this problem Pfl108 1 1715 Bsa l 1766 Sal 2177 Amp* Xmn 1 2294 AlwN I 1217 ORI pUC18-207 -2700 bp Ssp 1 2501 Afl III 806 lacZ promoter Sap / 683 Nde l 183 MCS 454 396 EcoRI Kpn Bam HI Xba l SalI Pst l Hind III Sac Notl PstI 7000 EcOR I500 CamR ORI Bam HI 1500 Hind IIl 6034 Sal 1 1963 Bam HI 6029 Not I 6017 Xbal 1972 pCamR-GFP 8000 bp Bsa 1982 - Not I 1995 Sac / 6011 GFP Sal I 3500 Bam HI 4023 1) How large is the GFP gene? Express your answer in base pairs. (1 mark)

Explanation / Answer

Length of  pCamR-GFP = 8000 bp

GFP ranges from 1995 bp to 6011 bp. So a total of 4016 bp.

Length of pCamR without GFP = 8000 - 4016 = 3984 bp

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