Assume a starting culture density of 120 cells/mL and a generation time of 30 mi
ID: 2871111 • Letter: A
Question
Assume a starting culture density of 120 cells/mL and a generation time of 30 min/generation. Describe the growth of the culture by filling in the culture densities at the indicated time points. How many generations have passed at the indicated time points? The bacteria reach the stationary phase of their growth curve when their cell density reaches 1010 cells/ml. How long will it take them to reach the stationary phase in terms of time (hr) and generations? Please answer by completing Table 2, all yellow cells should be completed.
Table 2 Culture density as a function of incubation time
Time (hr)
Culture
density (cells/ml)
Generations
0
1.20x102
0
1
2
3
...
...
...
Stationary phase 1010 cells/mL
Time (hr)
Culture
density (cells/ml)
Generations
0
1.20x102
0
1
2
3
...
...
...
?Stationary phase 1010 cells/mL
?Explanation / Answer
Culture density = 120 cells/mL
Generation time= 30 min/generation
C=C0 (t+1) ; G=t/G
at t=0 ; C=1.2X102 ; Generation = 0
at t=1 ; C=2.4X102 ; Generation = 2
at t=2 ; C=3.6X102 ; Generation = 4
at t=3 ; C=4.8X102 ; Generation = 6
at t=7 ; C=1010 cells/ml ; Generation = 12
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