What are two methods to localize a protein in a cell? What is a reporter? What i
ID: 319276 • Letter: W
Question
What are two methods to localize a protein in a cell?
What is a reporter? What is a reporter gene? What are two examples of reporter genes?
What is a transcriptional fusion? What is a translational fusion? How are these fusion constructs made?
What is PCR? What is typically found in a PCR reaction? How does temperature affect PCR reactions?
What are two sources of template DNA for PCR? How is cDNA generated?
What do restriction nucleases do?
What is a ligation? What is a transformation?
How are transgenic mice expressing either a translational or transcriptional fusion generated?
Explanation / Answer
Ques-1: What are two methods to localize a protein in a cell?
Answer: Immunohistochemistry
Fluorescent imaging of GFP expression
Fusion protein tags
The above methods are meticulously used for detection specific protein expression localized in a cell. The antibody labeling via primary and secondary immune labeling is going to be useful to find a specific protein. The labeled protein can be detected by fluorescent imaging
Ques-2: What do restriction nucleases do?
What is a ligation? What is a transformation?
Restriction nucleases are the enzymatic proteins acts a molecular scissors useful to cut specific gene sequences in the plasmid or cell genome during recombinant DNA technology
Ques-3: Ligation is the process of addition gene sequences to the specific gene so that a recombinant gene is produced useful for transformation
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