When calculating the concentration of bacteria in a liquid culture, you should p

Question

When calculating the concentration of bacteria in a liquid culture, you should plate multiple dilutions of your culture then count the colonies that develop on only one of those plates.  The plate that you count should contain between 30 and 300 colonies.  Should you avoid using plates with fewer than 30 or more than 300 colonies because some error in your technique led to those numbers of colonies, or because using fewer than 30 and more than 300 colonies can lead to an error in your calculations?  Explain your answer.

Explanation / Answer

The growth of fewer than 30 colonies on a plate results in astatistically invalid count, and more than 300 colonies on a platecan cause interference among cells and colonies duringgrowth. Found On googlebooks Microbiology 3rdEdition By Daniel V. Lim Page126       Found On googlebooks Microbiology 3rdEdition By Daniel V. Lim Page126      

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