Cell Biology Please Need Help With Problem ( Part 3) Please and Thank you in adv

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Cell Biology

Please Need Help With Problem ( Part 3)

Please and Thank you in advance!

3 . (2.0 points) You are doing tests on the signal transduction of cells in order to learn experimental set-up procedures. These cells have a G-Protein Coupled Receptor (GPCR). When the GPCR is activated by its ligand, the cellular response is a rise of cAMP levels in the cytosol. You stimulate the system with ligand at the (*) The resulting cAMP levels inside the cell are shown in the graph below. . A. You treat the cells with protease, then add ligand (at time *). Which graph (1, 2 or 3 above) would best represent your expected result? Explain why you chose this graph. What is happening at the molecular level? . B. You inject GMP-PNP (a non-hydrolyzable analog of GTP) into the cells, then add ligand (at time *) Which graph (1 , 2 or 3 from part a) would best represent the expected result? Explain why you chose this graph. What is happening at the molecular level?

Explanation / Answer

1. graph 2 will be appropriate if u add the protease before the ligand. bcos protease will inhibit the protein as whole which include Gprotein

2. Graph 3 will be appropriate bcos adding analog og Gprotein will steaply increase the cAMP level

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