What region of the thiolase protein contains the peroxisomal targeting sequence?
ID: 49633 • Letter: W
Question
What region of the thiolase protein contains the peroxisomal targeting sequence? Explain your answer. ?
2.)
Acetylcholine binds to a GPCR on heart muscle, making the heart beat more slowly. The activated receptor stimulates a G protein, which opens a K+ channel in the plasma membrane, as shown in the Figure.
Which of the following would enhance this effect of the acetylcholine? Explain
?a. addition of a high concentration of a non-hydrolyzable analog of GTP
?b.addition of a drug that prevents the ? subunit from exchanging GDP for GTP
?c. mutations in the acetylcholine receptor that weaken the interaction between the receptor and acetylcholine?
d. mutations in the acetylcholine receptor that weaken the interaction between the receptor and the G protein
The numbers above each protein indicate the amino acids from thiolase present in the hybrid protein.?+ indicates growth on medium lacking histidine;?– indicates lack of growth on medium lacking histidine.
A. How would you explain this pattern given that KDEL is supposed to be an ER-specific sorting sequence?
To analyze the results further, fractions of different organelles and the cytoplasm were collected from cells expressing this KDEL-containing GFP construct and then examined on Western blots using antibodies against GFP (27 kDa) and protein disulfide isomerase (PDI), a resident-rough ER (RER) protein of approximately 55 kDa.
The blot confirms the presence of GFP exclusively in the cytoplasm, and as expected a PDI signal in the RER fraction.
B. How would you explain the PDI band, albeit weak, in the Golgi fraction?
C. Given the function of PDI proteins, what would you expect if both alleles of a PDI gene were knocked out in mice? [Think about what they do and how essential they are, if at all]
The antibodies used above do not detect any signals in the nuclear fraction, which indicates their specificity or the fact that no proteins were isolated and loaded in the nuclear fraction lane.
D. What antibody could be used to show there were nuclear proteins present in this sample? Just find a valid example there are many correct answers]. Justify your choice.
Explanation / Answer
Answer 1) As we see in the diagram; there are total 200 amino acids between Hydrolase and HDH.
considering all the above facts, we can conclude that the peroxisomal targeting sequence for thiolase enzyme in yeast must be present within the region comprising of 100-125amino acids.
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