What would happen if the TLC plate is immersed in the mobile phase such that the

Question

What would happen if the TLC plate is immersed in the mobile phase such that the level of the mobile phase is higher than the level of the samples on the TLC plate? What would happen to the movement of a solute "spot" if a mobile phase of too low a polarity is used? What would happen if the mobile phase is too high a polarity? (Assume the TLC plate is a silica plate). Rank the following compounds in order of increasing Rf values on a silica TLC plate: octane, 1-octanol, 1-chlorooctane, and octanoic acid. Why are pencils used for writing on TLC plates and not pens? What would occur if a pen is used?

Explanation / Answer

8.4 Thin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing. TLC plate is very fragile and easily chips. Plates must be handled with care. Pencils are used for writing on TLC plates because inks commonly travel up the plate with the solvent. If a pen is used then ink will travel up the plate with the solvent.

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