When a microscale extraction is carried out, which layer should be removed from

Question

When a microscale extraction is carried out, which layer should be removed from a centrifuge (or a conical vial) first in order to separate the layers? aqueous layer should be always removed first and placed in a different vial organic layer containing a product should be always removed first and placed in a different vial bottom layer (either aqueous or organic) should be removed first and placed in a different vial top layer (either aqueous or organic) should be removed first and placed in a different vial

Explanation / Answer

Organic layer with product is removed first. This is repeated in multiple stages by extraction with organic layer. Later on all this extracts kept in separate voils are vapourised to remove the organic layer

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