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Glycosylphosphatidylinositol anchors are added to proteins: on the C-terminus af

ID: 56472 • Letter: G

Question

Glycosylphosphatidylinositol anchors are added to proteins:

on the C-terminus after translation into the E.R. via a SRP-SRP receptor mechanism

on the C-terminus following translation in the cytoplasm via a SRP-SRP receptor independent mechanism

on the C-terminus by BIP

that are localized within the nuclear envelope

on the C-terminus after translation into the E.R. via a SRP-SRP receptor mechanism

on the C-terminus following translation in the cytoplasm via a SRP-SRP receptor independent mechanism

on the C-terminus by BIP

that are localized within the nuclear envelope

Explanation / Answer

Glycosylphosphatidylinositol is a glycolipid, which is attached to protein at C-terminus during post-translational modification. The signal sequence is associated with GPI-linked proteins, which directs the protein to ER. The protein inserted into the ER is attached to ER membrane by hydrophobic C terminus.

The proteins are synthesized on free cytosolic ribosomes and their posttranslational modifications occur in ER. Thus, the proteins are incorporated into the ER. The incorporation of protein requires simple signal sequences and certain chaperones in the ER such as BiP but does not require SRP. The BiP pulls the polypeptide chain into the ER through the channel. Thus, the binding of polypeptide chains to BiP is needed to drive the posttranslational translocation of proteins into the ER. In contrast, the co-translational translocation of growing polypeptide chains is driven directly by the process of protein synthesis.

Hence, the correct option among the following is 3rd- on the C-terminus by BIP.

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