Question You are working in the lab and discover a new factor (X) that appears t

Question

Question

You are working in the lab and discover a new factor (X) that appears to play some important role in wound healing. You are provided with the information that each receptor only binds a single X. In order to better characterize the interaction of X with a putative receptor, you obtain dose-response and binding data on X and obtain the following results:

[X]                      Bound X (on 10^7)           Relative Rate of Proliferation

0                             0                                               1

10^-12 M               0.2 pmols                                 1.7

10^-11 M               1.8 pmols                                  4.2

10^-10                   6.0 pmols                                 5.2

10^-9                     10 pmols                                    5.4

10^-8                      12 pmols                                    5.4

These are the data you wish to report:

1. Approximate numbers of X receptor molecules per cell

2. Half-maximal Dose

3. Apparent Kd for the X receptor

4. Any other details that you think might be important

Write a very concise report containing all the information asked and a short paragraph describing why do you think these findings might be interesting.

How would you graph this problem?

Explanation / Answer

one receptor binds to one X. The value at 6 pmols of X is 5.2 and after that it becomes constant. This means that now, if more X is added, there are no more receptors left to bind with X. So, number of receptors should be equal to that bound concentration of X at which relative rate of proliferation stops increasing further. So, approximate number of X receptor molecules per cell should be 6- 10pmols.

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