Question You are working in the lab and discover a new factor (X) that appears t
ID: 59456 • Letter: Q
Question
Question
You are working in the lab and discover a new factor (X) that appears to play some important role in wound healing. You are provided with the information that each receptor only binds a single X. In order to better characterize the interaction of X with a putative receptor, you obtain dose-response and binding data on X and obtain the following results:
[X] Bound X (on 10^7) Relative Rate of Proliferation
0 0 1
10^-12 M 0.2 pmols 1.7
10^-11 M 1.8 pmols 4.2
10^-10 6.0 pmols 5.2
10^-9 10 pmols 5.4
10^-8 12 pmols 5.4
These are the data you wish to report:
1. Approximate numbers of X receptor molecules per cell
2. Half-maximal Dose
3. Apparent Kd for the X receptor
4. Any other details that you think might be important
Write a very concise report containing all the information asked and a short paragraph describing why do you think these findings might be interesting.
How would you graph this problem?
Explanation / Answer
1)
Based on the given data,
The receptor and ligands binds in a 1:1 stoichiometry (one ligand per one receptor). Therefore,
10-8 M × 6×1023
= 6×1015 receptors / 107 cells,
So,
6×1015 / 107
X = 6×108 receptors/ cell.
2)
The given data is:
The dosage response curve is as follows:
The Half-maximal Dose is:
y = min + ((max-min)/(1+(X/EC50)-Hill slope))
Where
Therefore =
y = 0 + ((12-0)/(1+(6×108/6×1010)-3.18))
= 5.2×10-6
Therefore, the Half maximum does = 5.2×10-6.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.