Question You are working in the lab and discover a new factor (X) that appears t

Question

Question

You are working in the lab and discover a new factor (X) that appears to play some important role in wound healing. You are provided with the information that each receptor only binds a single X. In order to better characterize the interaction of X with a putative receptor, you obtain dose-response and binding data on X and obtain the following results:

[X]                      Bound X (on 10^7)           Relative Rate of Proliferation

0                             0                                               1

10^-12 M               0.2 pmols                                 1.7

10^-11 M               1.8 pmols                                  4.2

10^-10                   6.0 pmols                                 5.2

10^-9                     10 pmols                                    5.4

10^-8                      12 pmols                                    5.4

1. What is the Apparent Kd for the X receptor

2. Are there Any other details that you think might be important

Explanation / Answer

Kd = Concentration of ligand X concentration of receptor / concentration of ligand bound receptor

Conc of ligand is 200 X107

Kd = 200 X107 x 10-10 / 6 X 10-12

= 200 X 10-9 / 6

= 33.3 X 10-9

2. The concentration of Kd is very less. This shows that the Factor X has high affinity for the receptor.

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